Wolfgang Kuebler

Keenan Research Centre for Biomedical Science of St. Michael's
Co-Director, Critical Illness and Injury Research Centre
Professor, Depts. of Surgery and Physiology, Univ. of Toronto
Professor, Dept. of Physiology, Charité - Universitätsmedizin Berlin
endowed by the German Heart Institute Berlin

Research Synopsis

Keywords: Acute lung injury, pulmonary hypertension, lung edema, intravital microscopy.

Detailed Description: Our interest focuses on translational research in pulmonary and microvascular physiology and pathophysiology, in that we aim to integrate basic research on cell signalling and communication pathways to the level of the intact organ and organism. This approach allows us to identify new basic pathomechanisms in complex disease models such as acute lung injury, lung edema, or pulmonary hypertension. We then aim to apply this knowledge for the development and preclinical testing of new therapeutic strategies for the treatment of these diseases.

A special methodological focus of our work is the use of intravital microscopy and real-time fluorescence imaging techniques in intact organs or life animal models, which we combine with both cell and molecular biology techniques as well as physiological measurements.

METHODS USED

Cell and tissue culture: Endothelial cells, smooth muscle cells.

Procedures: Elisa, Gene Expression Analysis, Immunohistochemistry, qRT-PCR, RT-PCR, Signal Transduction Characterization, siRNA, Western Blot

EQUIPMENT USED

Benchtop Centrifuge, Blotting Aparatus, Calcium Imaging System, Confocal Microscope, Culture Hood, Culture Incubators.Flurorescence Microscope, Micropipette Puller, Microwave Oven, Monochromator, Water Baths

PRESENT TRAINEES

Laura Michalik
Siegfried Breitling
Adrienne Krauszman
Sarah Weidenfeld
Mark McVey
Mazharul Maishan

PRESENT COLLABORATIONS

Within the Department of Physiology:
Steffen-Sebastian Bolz
Haibo Zhang

Outside the Department of Physiology:
Thomas Walther Univ. of Mannheim, Germany
Stefan Uhlig Univ. of Aachen, Germany
Rubin Tuder Univ.of Denver, US
Rory Morty Univ of Giessen, Germany
Martin Witzenrath Univ of Berlin, Germany

Committee member/Officer of national or international scientific organization:
Past-Chair, Respiration Section, American Physiological Society
Chair, Section Advisory Committee, American Physiological Society
Member, Council, American Physiological Society
Member, Strategic Committee, European Society for Microcirculation
Member, Program Committee, Pulmonary Circulation Assembly, American Thoracic Society
Chair, Scientific Advisory Committee, Ludwig-Boltzmann-Institute for Lung Vascular Research, Austria

PRESENT GRANT COMMITTEES SERVED ON
Agency: CIHR
Commitee: College of Reviewers for CIHR Foundation Scheme, Phase 1

Recent Publications

Reviews:

http://www.ncbi.nlm.nih.gov/pubmed?term=Kuebler%20WM%20AND%20reviews

Appointments

Cross-Appointed
Primary: Surgery