John Laffey MD
Cardiovascular and Respiratory Platform
Contact Info
T: (416) 864-5071
F: (416) 864-6015
Dept of Anesthesia, St Michaels’s Hospital, 30 Bond Street
Toronto, ON, M5B 1W8
Primary: Anaesthesia
Research Interests
Acute lung injury, acute respiratory distress syndrome, Pee-clinical animal models, sepsis, ventilation induced lung injury, cell theory, gene based therapy


Research Synopsis

Research Interests:
Cell based therapies for Acute Respiratory Distress Syndrome
Gene based therapies for Acute Respiratory Distress Syndrome
Effects and mechanisms of action of CO2 in the Critically Ill
Role of sepsis and mechanical ventilation in pathogenesis of Acute Respiratory Distress Syndrome
Acute pain management following major Surgery
Evaluation of medical equipment, particularly novel Airway-related devices.

Detailed Description: My major laboratory research interests are centred on investigation of the pathophysiology of, and development of therapeutic strategies for Acute Respiratory Distress Syndrome (ARDS), a devastating disease which causes severe respiratory failure in critically ill patients, and for which there are currently no specific therapies.

A major research theme is the therapeutic potential of cell and gene based therapies for ARDS. A second major interest is the study of the effects and mechanisms of action of hypercapnia and acidosis in the setting of Critical Illness.

I have clinical research interests in the fields of Anesthesia and Critical Care Medicine. Specific interests include investigation of novel approaches to postoperative analgesia, the evaluation of novel airway devices, and translational studies in the critically ill.


Cell and tissue culture: Human pulmonary epithelial cell cultures, mesenchymal stromal cells

Procedures: Assessment of lung function, assessment of hemodynamic function, Elisa, immunohistochemistry, in vitro electrophysiology, in vivo electrophysiology, qRT-PCR, RT-PCR, vessel cannulation, western blot


Amplifier, benchtop centrifuge, blotting apparatus, culture hood, fresh tissue sectioning systems, infusion apparatus, low- and high-speed centrifuge, low and ultralow freezers, plate reader, stirrer/hot plate, water baths


Rajdeep Gill (MSc Physiology)


Within the Department of Physiology:
Brian Kavanagh

Committee member/Officer of national or international scientific organization:
Deputy Chair, Acute Respiratory Failure Section, European Society of Intensive Care Medicine.
Deputy Chair, Irish Critical Care Trials Group.
Member, Research Committee, European Society of Intensive Care Medicine.
Member, Editorial Board of Intensive Care Medicine Experimental.
Member, Editorial Board of Journal Perioperative Medicine.
Member, Anaesthesia and Perioperative Medicine Section, Faculty of 1000 Medicine

Publications and Awards

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Recent Publications