Keywords: Development, prefrontal cortex, electrophysiology, calcium imaging, neuropharmacology, molecular manipulations, acetylcholine receptors, serotonin (5-HT) receptors.
Detailed Description: The Lambe Lab research team focuses on the prefrontal cortex, a brain region critical for attention as well as the regulation of anxiety. We study the development of prefrontal cortical neurons and circuitry using the tools of electrophysiological recording and multiphoton imaging. These techniques allow us to assess functional receptors and local circuitry at different points in development and determine how these are changed by molecular, pharmacological, and behavioural manipulations. Such information is important to identify vulnerabilities in the development of brain circuitry and to design novel therapeutic interventions for attention and anxiety disorders.
Cell and tissue culture: Brain slice, neurons.
Procedures: Electrophysiology, immunohistochemistry, in-vitro electrophysiology, intracellular injection, patch clamp, signal transduction characterization, stereotaxic brain surgery, voltage clamp, western blot
Amplifier, analytical balances, calcium imaging system, digidata, electrophysiology rig, fluorescence microscope, fresh tissue sectioning systems, micropipette puller, microwave oven, motorized micromanipulators, real-time/thermocycler, stimulator, vibratome, water baths.
Dr. Derva Sargin
Within the Department of Physiology:
Dr. Zhong-Ping Feng
Dr. Scott Heximer
Dr. Mike Salter
Outside the Department of Physiology:
Dr. Mariella DeBiasi, Baylor College of Medicine, Houston Texas, USA
Dr. Paul Fletcher, University of Toronto, Toronto ON, Canada
Dr. Jay Gingrich, Columbia University, New York NY, USA
Dr. Raad Nashmi, University of Victoria, Victoria BC, Canada
Dr. Lucy Osborne, University of Toronto, Toronto ON, Canada
Dr. Marina Picciotto, Yale University, New Haven CT, USA
Dr. Vidita Vaidya, Tata Institute of Fundamental Biology, Mumbai, India
Dr. Cynthia Weickert, University of New South Wales, Sydney, Australia