1. Genome wide gene trapping and mutagenesis in zebrafish
2. Large scale high throughput chemical genetic screening for in vivo drug discovery using zebrafish embryos
3. Functional genomics and disease modeling
Keywords: Zebrafish, disease modeling, high throughput, mutagenesis, gene trapping, chemical genetics, drug development.
Cell and tissue culture: Brain slice, cardiomyocytes, endothelial cells, pancreas cells.
Procedures: Behavioural tests, gene expression analysis, immunohistochemistry, qRT-PCR, RT-PCR, western blot.
Amplifier, analytical balances, benchtop centrifuge, blotting apparatus, confocal microscope, culture hood, culture incubators, digital microscope, dissecting microscope, electrometer, fluorescence microscope, microwave oven, plate reader.