Research InterestsInfection, immunity, lung injury, defensin biology, phagocytosis, sepsis, cytokine, toll-like receptors.
Degrees: Ph.D. 1995, M.D. 1988
Affiliations: St. Michael's Hospital
My major research interests relate to lung infection and immunity and sepsis. Specifically, my lab is working on:
- Human neutrophil peptides in host defense and lung injury: To examine the molecular mechanisms by which human neutrophil peptides impair phagocytic function and inflammatory responses in human cystic fibrosis and other lung diseases, and develop therapeutic approaches targeting the mechanisms of action of human neutrophil peptides.
- Novel LPS receptor: To identify alternative LPS receptors in lung cells and alveolar macrophages independent of TLR4.
- Cytokine and bacterial interaction: To examine the mechanisms by which cytokines kill and promote bacterial proliferation. My laboratory uses a number of model systems including in vitro primary human and murine cells and transformed human and murine cell lines, ex vivo lung explants and various animal models.
- I have been involved in clinical trials related to biomarkers of inflammation.
Cell and tissue culture: Endothelial cells.
Procedures: Elisa, gene expression analysis, immunohistochemistry, isolated vessel preparation, mass spectrometry, microarrays, proteomics, qRT-PCR, RT-PCR, signal transduction characterization, siRNA, vagotomy, western blot.
Benchtop centrifuge, blotting apparatus, confocal microscope, culture hood, culture incubators, fluorescence microscope, gel apparatus, infusion apparatus, low- and high-speed centrifuge, low and ultralow freezers, mass spectrometer, micropipette puller, microwave oven, mini vortexer, plate reader, real-time/thermocycler, setups for electropherosis, stirrer/hot plate.
Alice Luo PhD candidate
Diana Islam PhD candidate
Ustina Huh MSc candidate
Florence Morriello MSc candidate
Alice Grassi Post Doctoral Fellow
Manshu Li PhD exchange student
Junbo Zheng PhD exchange student
Within the Department of Physiology:
Outside the Department of Physiology:
Arthur Slutsky Critical Care Medicine/UofT
Jane Batt Respiratory Medicine/UotT
Warren Lee Critical Care Medicine/UofT
John Marshall Critical Care Medicine/UofT
Ian Tannock Oncology/UofT
Gordon Winocur Baycrest Institute Toronto
Janette Vardy Sydney Cancer Centre/University of Sydney
Rolf Hubmayr Respiratory Medicine/Mayo Clinic
Nanshn Zhong Respiratory Medicine/Guangzhou Medical University
Committee member/officer of national/international scientific organizations
Reappointment committee, SMH
Appointment committee, IMS
Associate Editor, Intensive Care Medicine
PRESENT GRANT COMMITTEES SERVED ON
Committee: Doctoral Scholarship committee
Publications and Awards