gramolini
Anthony GramoliniPhD
Associate Professor
Cardiovascular and Respiratory Platform

Location

Ted Rogers Centre for Heart Research, Translational Biology and Engineering Program, U of T
661 University Ave. 14th Floor, Rm 1437
Toronto
ON, M5G 1M1

Research Interests

Mechanisms of dilated cardiomyopathy; Ca2+ regulation in skeletal and cardiac muscle; formation of specialized protein domains in muscle; cardiovascular proteomics.

Accepting

None

Appointments

Heart and Stroke, Richard Lewar Centre
Toronto General Hospital Research Institute (Cell and Molecular Biology; Advanced Diagnostics).

Research/Teaching

Research Synopsis:

Keywords: Ca2+ cycling, cardiac muscle, dilated cardiomyopathy, cell biology, proteomics, biomarkers, heart failure.

Detailed Description:
A major interest of our lab is to investigate the cellular mechanisms involved in the regulation of calcium cycling at the level of sarcoplasmic reticulum and its role in cardiac disease. For these experiments, we apply conventional biochemical and molecular biology assays, digital confocal imaging, and mass spectrometry based proteomic studies. Model systems include: tissue explants, established cell lines, primary cell cultures and transgenic animals. These techniques serve as valuable experimental tools to investigate heart disease pathways, cellular protein sorting and Ca2+ cycling.

METHODS USED

Cell and tissue culture: Cardiomyocytes, endothelial cells, smooth muscle cells.

Procedures: Cell biology, confocal imaging, HPLC, gene expression analysis, immunohistochemistry, mass spectrometry, mircoarrays, proteomics, qRT-PCR, RT-PCR, siRNA, transgenic mice, western blot.

EQUIPMENT USED

Calcium imaging system (Olympus), confocal microscope (Leica LSM IRBE), culture hood, culture incubators, digital microscope (Zeiss structured illumination (SIM), fluorescence microscope, HPLC (Akta), Kodak documentation system, low- and high-speed centrifuge, mass spectrometer (LTQ Orbitrap, TSQ Vantage), plate reader (Perkin Elmer), setups for electropherosis.

PRESENT TRAINEES

Jake Cosme, MSc candidate
Diana Buchsbaum, MSc candidate
Wenping Li, Tech II
Aaron Wilson, Tech II
Dr. Allen Teng, PDF
Dr. Dingyan Wang, research associate

PRESENT COLLABORATIONS

Within the Department of Physiology:
Peter Backx

Outside the Department of Physiology:
Gordon Keller, UHN
Peter Liu, UHN
Thomas Kislinger, UHN
Mansoor Husain, UHN
Ian Scott, Sick Kids
Seema Mital, Sick Kids
Robert Hamilton, Sick Kids
Jack Greenblatt, CCBR
Andrew Emili, CCBR
Gary Bader, CCBR
Jason Moffat, CCBR
Igor Stagljar, CCBR
Benoit Bruneau, USCF Gladstone

Courses:

PSL301, PSL350, PSL498 (Coordinator), PSL462/1462, PSL1040, PSL1067, JCV3060, JCV3062

Publications and Awards

Recent Publications

  1. L.Zhang, X.Chen, M.Nghiem, M.Moon, F.Dawood, M.Chen, P.Sharma, A.O.Gramolini, J.M.Penninger and P.P.Liu (2014) HACE1 maintains cardic cytoskeleton homeostasis by regulating ubiquitin-selective autophagy. Nature Communications 5, 3430
  2. D.-Y. Wang, C. Abbasi, S. El-Rass, J. Y. Li, F. Dawood, K. Naito, P. Sharma, A. Wilson, S. Yokoe, N. Bousette, X.-Y. Wen, P.P. Liu and A.O. Gramolini. (2014) ERp44 deficiency results in aberrant Ca2+ homeostasis, ER stress-induced apoptosis, and cardiomyopathy. J Am Heart Assoc 3, e001018  . *Article profiled in JAHA.
  3. V.A. Fajardo, E. Bombardier, C. Vigna, T. Devji, D. Bloemberg, D. Gamu, A.O. Gramolini, J. Quadrilatero, A.R.Tupling (2014) Co-expression of SERCA Isoforms, Phospholamban and Sarcolipin in Human Skeletal Muscle Fibers. PloS One 8, e94304.
  4. Sun M, Ouzounian M, de Couto G, Chen M, Yan R, Fukuoka M, Li G, Moon M, Liu Y, Gramolini A, Wells GJ, Liu PP Cathepsin-L ameliorates cardiac hypertrophy through activation of the autophagy-lysosomal dependent protein processing pathways..J Am Heart Assoc. 2013 Apr 22;2(2):e000191. doi: 10.1161/JAHA.113.000191.PMID
  5. P. Sharma, N. U.Nair, W. Li, A. Dong, T. Miyake, A. Wilson, T.Ryan, T. Kislinger, M. Ikura, S.Dhe-Paganon, and A.O.Gramolini (2012) Structural Determination of the Phosphorylation Domain of the Ryanodine Receptor.  Fed Euro Biol Soc  (FEBS) J 20,3952-64.
  6. P.J. Rugg-Gunn, B.J. Cox, F. Lanner, P.Sharma, V.Ignatchenko, J.Garner, A.O.Gramolini, J.Rossant and T. Kislinger (2012) Identification of cell-surface proteins enables isolation of viable lineage progenitor cells directly from mouse blastocysts using flow cytometry. Developmental Cell 22,887-901
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